SrasV1, France, Analysis, bibRecord, 000274

The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles

Identifieur interne : 000274 ( France/Analysis ); précédent : 000273; suivant : 000275

The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles

Auteurs : Y. L. Siu [Hong Kong] ; K. T. Teoh [Hong Kong] ; J. Lo [Hong Kong] ; C. M. Chan [Hong Kong] ; F. Kien [Hong Kong] ; N. Escriou [France] ; S. W. Tsao [Hong Kong] ; J. M. Nicholls [Hong Kong] ; R. Altmeyer [Singapour] ; J. S. M. Peiris [Hong Kong] ; R. Bruzzone [Hong Kong] ; B. Nal [Hong Kong]

Source :

Journal of virology [ 0022-538X ] ; 2008.

RBID : Pascal:08-0522049

Descripteurs français

KwdFr :
- Animaux, Assemblage viral, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Microscopie électronique à transmission, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (génétique), Protéines de la matrice virale (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Virosomes (métabolisme), Virosomes (ultrastructure), Virus du SRAS (physiologie).
MESH :
- génétique : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Virosomes.
- physiologie : Virus du SRAS.
Pascal (Inist)
- Animaux, Assemblage viral, Cellules Vero, Coronavirus, Glycoprotéine de spicule des coronavirus, Humains, Microscopie électronique à transmission, Protéine, Libération, Particule type viral, Virologie, Syndrome respiratoire aigu sévère, Virosomes.

English descriptors

KwdEn :
- Animals, Chlorocebus aethiops, Coronavirus, Humans, Membrane Glycoproteins (metabolism), Microscopy, Electron, Transmission, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Protein, Release, SARS Virus (physiology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Virology, Virosomes (metabolism), Virosomes (ultrastructure), Virus Assembly, Virus like particle.
MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- chemical , metabolism : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Virosomes.
- physiology : SARS Virus.
- chemical , ultrastructure : Virosomes.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron, Transmission, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Assembly.

Abstract

The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-00543224

Affiliations:

Links to Exploration step

Pascal:08-0522049

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus</term>
<term>Humans</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microscopy, Electron, Transmission</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Protein</term>
<term>Release</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (genetics)</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Virology</term>
<term>Virosomes (metabolism)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus Assembly</term>
<term>Virus like particle</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Microscopie électronique à transmission</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (génétique)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Virosomes (métabolisme)</term>
<term>Virosomes (ultrastructure)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
<term>Virosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Protéines nucléocapside</term>
<term>Virosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Virosomes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Microscopy, Electron, Transmission</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Virus Assembly</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules Vero</term>
<term>Coronavirus</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Microscopie électronique à transmission</term>
<term>Protéine</term>
<term>Libération</term>
<term>Particule type viral</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virosomes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The production of virus-like particles (VLPs) constitutes a relevant and safe model to study molecular determinants of virion egress. The minimal requirement for the assembly of VLPs for the coronavirus responsible for severe acute respiratory syndrome in humans (SARS-CoV) is still controversial. Recent studies have shown that SARS-CoV VLP formation depends on either M and E proteins or M and N proteins. Here we show that both E and N proteins must be coexpressed with M protein for the efficient production and release of VLPs by transfected Vero E6 cells. This suggests that the mechanism of SARS-CoV assembly differs from that of other studied coronaviruses, which only require M and E proteins for VLP formation. When coexpressed, the native envelope trimeric S glycoprotein is incorporated onto VLPs. Interestingly, when a fluorescent protein tag is added to the C-terminal end of N or S protein, but not M protein, the chimeric viral proteins can be assembled within VLPs and allow visualization of VLP production and trafficking in living cells by state-of-the-art imaging technologies. Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Hong Kong</li>
<li>Singapour</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Siu, Y L" sort="Siu, Y L" uniqKey="Siu Y" first="Y. L." last="Siu">Y. L. Siu</name>
</noRegion>
<name sortKey="Bruzzone, R" sort="Bruzzone, R" uniqKey="Bruzzone R" first="R." last="Bruzzone">R. Bruzzone</name>
<name sortKey="Chan, C M" sort="Chan, C M" uniqKey="Chan C" first="C. M." last="Chan">C. M. Chan</name>
<name sortKey="Kien, F" sort="Kien, F" uniqKey="Kien F" first="F." last="Kien">F. Kien</name>
<name sortKey="Lo, J" sort="Lo, J" uniqKey="Lo J" first="J." last="Lo">J. Lo</name>
<name sortKey="Nal, B" sort="Nal, B" uniqKey="Nal B" first="B." last="Nal">B. Nal</name>
<name sortKey="Nicholls, J M" sort="Nicholls, J M" uniqKey="Nicholls J" first="J. M." last="Nicholls">J. M. Nicholls</name>
<name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name>
<name sortKey="Teoh, K T" sort="Teoh, K T" uniqKey="Teoh K" first="K. T." last="Teoh">K. T. Teoh</name>
<name sortKey="Tsao, S W" sort="Tsao, S W" uniqKey="Tsao S" first="S. W." last="Tsao">S. W. Tsao</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Escriou, N" sort="Escriou, N" uniqKey="Escriou N" first="N." last="Escriou">N. Escriou</name>
</region>
</country>
<country name="Singapour"><noRegion><name sortKey="Altmeyer, R" sort="Altmeyer, R" uniqKey="Altmeyer R" first="R." last="Altmeyer">R. Altmeyer</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles

The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles

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